human osteosarcoma cell line sjsa 1 Search Results


sjsa 1  (ATCC)
96
ATCC sjsa 1
Sjsa 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human osteosarcoma sjsa  (ATCC)
94
ATCC human osteosarcoma sjsa
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Human Osteosarcoma Sjsa, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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human os cell lines  (ATCC)
99
ATCC human os cell lines
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Human Os Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
human os cell lines - by Bioz Stars, 2026-03
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human osteosarcoma cell lines  (ATCC)
98
ATCC human osteosarcoma cell lines
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Human Osteosarcoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human osteosarcoma cell lines/product/ATCC
Average 98 stars, based on 1 article reviews
human osteosarcoma cell lines - by Bioz Stars, 2026-03
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bone crl 2098d sjsa 1 osteosarcoma pml blood ccl 240d hl  (ATCC)
90
ATCC bone crl 2098d sjsa 1 osteosarcoma pml blood ccl 240d hl
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Bone Crl 2098d Sjsa 1 Osteosarcoma Pml Blood Ccl 240d Hl, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bone crl 2098d sjsa 1 osteosarcoma pml blood ccl 240d hl/product/ATCC
Average 90 stars, based on 1 article reviews
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type culture collection ccl 171 nci n87 american type culture collection crl 5822 osa  (ATCC)
99
ATCC type culture collection ccl 171 nci n87 american type culture collection crl 5822 osa
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Type Culture Collection Ccl 171 Nci N87 American Type Culture Collection Crl 5822 Osa, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/type culture collection ccl 171 nci n87 american type culture collection crl 5822 osa/product/ATCC
Average 99 stars, based on 1 article reviews
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human osteosarcoma cell lines nos-10  (BioResource International Inc)
90
BioResource International Inc human osteosarcoma cell lines nos-10
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Human Osteosarcoma Cell Lines Nos 10, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human osteosarcoma cell lines nos-10 - by Bioz Stars, 2026-03
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human cancer cell lines  (ATCC)
99
ATCC human cancer cell lines
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Human Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cancer cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
human cancer cell lines - by Bioz Stars, 2026-03
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os cell lines  (ATCC)
96
ATCC os cell lines
(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) <t>SJSA</t> and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.
Os Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/os cell lines/product/ATCC
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os cell lines - by Bioz Stars, 2026-03
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human os cell lines (u2s2, saos-2, sjsa-1, and hos)  (iCell Gene Therapeutics)
90
iCell Gene Therapeutics human os cell lines (u2s2, saos-2, sjsa-1, and hos)
The qPCR result of seven lncRNAs in five OS highly <t>metastasis</t> and weakly metastasis cell lines. ( A ) AL512422.1. ( B ) AL008718.3. ( C ) AC006033.2. ( D ) AL357507.1. ( E ) AL360182.2. ( F ) C5orf66-AS1. ( G ) CEBPA-DT. *<0.05, **<0.01, ***<0.001, ****<0.0001.
Human Os Cell Lines (U2s2, Saos 2, Sjsa 1, And Hos), supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human os cell lines (u2s2, saos-2, sjsa-1, and hos)/product/iCell Gene Therapeutics
Average 90 stars, based on 1 article reviews
human os cell lines (u2s2, saos-2, sjsa-1, and hos) - by Bioz Stars, 2026-03
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Image Search Results


(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) SJSA and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.

Journal: Cancer research

Article Title: MDMX recruits UbcH5c to facilitate MDM2 E3 ligase activity and subsequent p53 degradation in vivo

doi: 10.1158/0008-5472.CAN-20-0790

Figure Lengend Snippet: (A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours, cell lysates were harvested and immunoprecipitated (IP) for MDM2 or MDMX, and blotted for indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated as in A and immunoprecipitated for MDM2 and MDMX and blotted for indicated proteins. (C) SJSA and MCF-7 cells were immunoprecipitated (IP) for MDM2 or MDMX, and immunoblotted with indicated antibodies. Input represents 5% of total cell lysate utilized for IP. (D) MEFs of indicated genotypes were treated with siRNA against Mdmx (MDMX) or non-specific sequences (NS) for 24 hour followed by treatment with 4-OHT for 2 hours. Cell lysates were harvested and immunoprecipitated for MDM2 and blotted for indicated proteins. (E) MDMX expressed in bacterial was analyzed by SDS-PAGE and Commassie Blue staining. (F) MDMX-UbcH5c complexes were resolved by SDS-PAGE and blotted for MDMX, GST, and UbcH5c.

Article Snippet: Cell lines The human osteosarcoma SJSA, osteosarcoma U2OS, and breast cancer MCF7 cells were obtained from the ATCC and came with comprehensive authentication and quality controls.

Techniques: Immunoprecipitation, SDS Page, Staining

The qPCR result of seven lncRNAs in five OS highly metastasis and weakly metastasis cell lines. ( A ) AL512422.1. ( B ) AL008718.3. ( C ) AC006033.2. ( D ) AL357507.1. ( E ) AL360182.2. ( F ) C5orf66-AS1. ( G ) CEBPA-DT. *<0.05, **<0.01, ***<0.001, ****<0.0001.

Journal: Cancer Management and Research

Article Title: A Novel Predictive Model Associated with Osteosarcoma Metastasis

doi: 10.2147/CMAR.S332387

Figure Lengend Snippet: The qPCR result of seven lncRNAs in five OS highly metastasis and weakly metastasis cell lines. ( A ) AL512422.1. ( B ) AL008718.3. ( C ) AC006033.2. ( D ) AL357507.1. ( E ) AL360182.2. ( F ) C5orf66-AS1. ( G ) CEBPA-DT. *<0.05, **<0.01, ***<0.001, ****<0.0001.

Article Snippet: Highly metastasis human OS cell lines (MNNG) and weakly metastasis human OS cell lines (U2S2, SAOS-2, SJSA-1, and HOS) were purchased from iCell (Shanghai, China).

Techniques: